Journal: APL Bioengineering

Article Title: Advanced hydrogel mesh platform with neural stem cells and human umbilical vein endothelial cells for enhanced axonal regeneration

doi: 10.1063/5.0244057

Figure Lengend Snippet: Gene expression of mNSCs and cytokine profiling of HUVEC. (a) Heatmap of 92 genes, including housekeeping genes, depicts the neurogenesis-associated mRNA expression in mNSC compared to BV2 cells. (b) A scatterplot indicates a greater than 12-fold difference in neurogenesis-related values between mNSCs and BV2 cells, with red spots emphasized. (c) Heatmap of 92 genes, including housekeeping genes, illustrates the mRNA expression associated with ECM and adhesion molecules in mNSCs compared to BV2 cells. (d) A scatterplot displays a more than 10-fold difference in ECM and adhesion molecule values between mNSCs and BV2 cells with red spots emphasized. (e) mRNA expressions of tPA and uPA in mNSCs and BV2 batches. (f) and (g) Cytokine expression profile of HUVEC to investigate the potential effects on ECM secretion.

Article Snippet: HUVEC was supplied by ATCC (Cat no. CRL-1730, Manassas, VA, USA) and BV2 cells were supplied by Cytion (Cat no. 305156, Eppelheim, Germany).

Techniques: Gene Expression, Expressing

Journal: APL Bioengineering

Article Title: Advanced hydrogel mesh platform with neural stem cells and human umbilical vein endothelial cells for enhanced axonal regeneration

doi: 10.1063/5.0244057

Figure Lengend Snippet: Transduction of GFP and mCherry into mNSCs, BV2, and HUVECs. (a) Representative images depict the vector designs used for lentiviral transduction of EGFP and mCherry. (b) Fluorescence microscopy of living cells showing EGFP expression in mNSCs and mCherry expression in BV2 and HUVEC cells, respectively. Scale bars measure 100 μ m. (c) Quantitative analysis of fluorescence intensities in individual cells using flow cytometry. The blue histogram represents the isotype control and the red histogram indicates positive cells. The percentage reflects gated population.

Article Snippet: HUVEC was supplied by ATCC (Cat no. CRL-1730, Manassas, VA, USA) and BV2 cells were supplied by Cytion (Cat no. 305156, Eppelheim, Germany).

Techniques: Transduction, Plasmid Preparation, Fluorescence, Microscopy, Expressing, Flow Cytometry, Control

Journal: APL Bioengineering

Article Title: Advanced hydrogel mesh platform with neural stem cells and human umbilical vein endothelial cells for enhanced axonal regeneration

doi: 10.1063/5.0244057

Figure Lengend Snippet: In vitro assay of mNSCs in co-culture system with BV2 and HUVEC. (a) Representative images show cell morphologies and adhesions on Matrigel prior to co-culture use. Scale bars measure 200 μ m. (b) Integration and migration of mNSCs within a HUVEC co-culture environment, not seen in BV2 cells. Scale bars measure 50 μ m. (c) mNSCs demonstrate high affinity for the vascular-like structure from which HUVEC were removed. Scale bars are 200 and 100 μ m.

Article Snippet: HUVEC was supplied by ATCC (Cat no. CRL-1730, Manassas, VA, USA) and BV2 cells were supplied by Cytion (Cat no. 305156, Eppelheim, Germany).

Techniques: In Vitro, Co-Culture Assay, Migration

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: Lipopolysaccharide‐induced hypothalamic inflammation in cancer cachexia‐anorexia is amplified by tumour‐derived prostaglandin E2

doi: 10.1002/jcsm.13093

Figure Lengend Snippet: (A) IL‐6 secretion of mHypoE‐N46 cells incubated with different concentrations LPS (0–1000 ng/mL). (B) IL‐ 6 secretion of BV2 cells incubated with different concentrations LPS (0–1000 ng/mL). (C) IL‐6 secretion of mHypoE‐N46 cells incubated with LPS‐supplemented culture medium (LPS), C26, LLC, KPC and 4,662 tumour secretome (C26, LLC, KPC, 4662) and combined with LPS‐supplemented culture medium (+LPS) and regular culture medium (DMEM). The IL‐6 values are given as mean concentration in pg/mL (A,B) and corrected for total protein concentration and presented as percentage of LPS (%). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001. One‐way ANOVA analysis of variance with post hoc Bonferroni's multiple comparison test.

Article Snippet: The mouse brain microglia (BV2) cell line was purchased from Banca Biologica e Cell Factory (Genova, Italy).

Techniques: Incubation, Concentration Assay, Protein Concentration, Comparison

Journal: Materials Today Bio

Article Title: Enhanced inhibition of neuronal ferroptosis and regulation of microglial polarization with multifunctional traditional Chinese medicine active ingredients-based selenium nanoparticles for treating spinal cord injury

doi: 10.1016/j.mtbio.2025.101758

Figure Lengend Snippet: TSIIA/TMP/APS@Se NPs regulate the polarization of BV2 cells and improve the inflammatory microenvironment to rescue PC12 cells in a co-culture system. (A–D) The ELISA quantitative analysis of the inflammatory cytokines in BV2 cells after different treatments. (E) Schematic illustration of the modulation of TSIIA/TMP/APS@Se NPs on BV2 polarization. (F) Protein expression images of iNOS and Arg-1 in BV2 cells after different treatment of nanoparticles. (G) Flow cytometry result of the BV2 cells to identify the M1 phenotype (F4/80, CD16/32 double positive) and M2 phenotype (F4/80, CD206 double positive) after incubation with F4/80, CD16/32, CD206. (H) Schematic illustration of a co-cultured system between PC12 cells and BV2 cells, indicating outcomes of diverse treatments with Se NPs. (I) The representative images of Live/Dead stains of PC12 cells after different treatments of the co-cultured system. Scale bar = 200 μm ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001, and ns ( P > 0.05) suggested no statistical difference.

Article Snippet: The immortalized murine microglial cell line BV2 is commonly used as a surrogate for primary microglia in CNS research [ ].

Techniques: Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry, Incubation, Cell Culture